Development and validated spectrophotometric method for the determination of Ceftriaxone in pharmaceutical preparations

The study involves the development of a simple, rapid, selective, and low-cost spectrophotometric method for determination ceftriaxone as pure in its pharmaceutical preparations. (CF) was diazotized by sodium nitrite NaNO2 solution presence hydrochloride acid HCl ice bath at range 0 to 5 ºC form diazonium salt, then coupled with 4,5-diphenylimidazole reagent (4,5-DPI) basic hydroxide purple color water–soluble azo dye result, that has maximum absorption λmax 550 nm opposite blank, optimum conditions. linearity process obeyed Beers law concentration 10 100 μg/mL correlation coefficient R2 0.9973. molar absorptivity 3772.08 L/mol.cm. detection limit quantification limits were 4.9425 16.475 μg/mL, respectively. Relative error precision determined be 2.8% 1.44%, resulting stoichiometry AZO (drug: reagent) formed found 1:1.